Use of conditioned medium for efficient transformation and cost-effective cultivation of Nannochloropsis salina

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The oleaginous microalga Nannochloropsis sp. has been spotlighted as a promising candidate in genetic engineering research for biodiesel production. However, one of the major bottlenecks in the genetic manipulation against Nannochloropsis sp. is low transformation efficiency. Based on the idea that they grow rapidly in broth culture, the effect of conditioned medium on colonization and transformation efficiency of Nannochloropsis salina was investigated. Cells grown on agar plates with 20-40% conditioned medium produced colonies that were approximately 2.3-fold larger than cells grown without conditioned medium. More importantly, the transformation efficiency was about 2-fold greater on plates with 30% conditioned medium relative to those without conditioned medium. In addition, FAME productivity in liquid cultures with 100% conditioned medium increased up to 20% compared with cultures of control medium. These results suggest that conditioned medium can be applied for efficient transformation and cost-effective cultivation of N. salina for biodiesel production.
Publisher
ELSEVIER SCI LTD
Issue Date
2015-04
Language
English
Article Type
Article
Keywords

CHLORELLA-VULGARIS; BIODIESEL PRODUCTION; GROWTH-MEDIUM; TRIACYLGLYCEROLS; PERSPECTIVES; GADITANA; CULTURES

Citation

BIORESOURCE TECHNOLOGY, v.181, pp.231 - 237

ISSN
0960-8524
DOI
10.1016/j.biortech.2015.01.040
URI
http://hdl.handle.net/10203/195775
Appears in Collection
CBE-Journal Papers(저널논문)
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