CRP detection from serum for chip-based point-of-care testing system

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Most of point-of-care testing (POCT) to improve facilitates in diagnosis, treatment, and monitoring of patients. POCT technique has still remained a quantitatively and accurately detective effect. In this article, we demonstrated that real human C-reactive protein (CRP) in serum was detected for a chip-based point-of-care testing application based on a nanogap-embedded field effect transistor (FET), and the results were compared with those obtained via the enzyme-linked immunosorbent assay (ELISA) method. The limit of detection (LOD), determined from the standard curve, was 0.1 ng/ml, which is comparable to that of commercialized ELISAs. We evaluated that an improved detection range (0.1 ng/ml to 100 ng/ml) was achieved by comparing with commercialized ELISA. Control experiments to determine selectivity and to discern false-positive/false-negative rates were also performed. This report is the first description of the detection of CRP in human serum using a silicon-based biosensor. (C) 2012 Elsevier B.V. All rights reserved.
Publisher
ELSEVIER ADVANCED TECHNOLOGY
Issue Date
2013-03
Language
English
Article Type
Article
Keywords

C-REACTIVE PROTEIN; SURFACE-PLASMON RESONANCE; FIELD-EFFECT TRANSISTOR; BIOSENSORS; ELISA

Citation

BIOSENSORS & BIOELECTRONICS, v.41, pp.322 - 327

ISSN
0956-5663
DOI
10.1016/j.bios.2012.08.047
URI
http://hdl.handle.net/10203/174531
Appears in Collection
EE-Journal Papers(저널논문)
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