DC Field | Value | Language |
---|---|---|
dc.contributor.author | Song, Su Jung | ko |
dc.contributor.author | Kim, Soon Jung | ko |
dc.contributor.author | Song, Min Sup | ko |
dc.contributor.author | Lim, Dae-Sik | ko |
dc.date.accessioned | 2010-02-08T05:58:34Z | - |
dc.date.available | 2010-02-08T05:58:34Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2009-11 | - |
dc.identifier.citation | CANCER RESEARCH, v.69, no.22, pp.8540 - 8544 | - |
dc.identifier.issn | 0008-5472 | - |
dc.identifier.uri | http://hdl.handle.net/10203/16499 | - |
dc.description.abstract | Aurora B is critically involved in ensuring proper cytokinesis and maintaining genomic stability. The tumor suppressor RASSF1A regulates cell cycle progression by regulating mitotic progression, G(1)-S transition, and microtubute stability. We previously reported that both Aurora A and Aurora B phosphorylate RASSF1A, and showed that phosphorylation of RASSF1A by Aurora A blocks the inhibitory function of RASSF1A toward anaphase-promoting complex-Cdc20. However, the role of Aurora B-mediated RASSF1A phosphorylation remains unknown. Here, we show that phosphorylation of RASSF1A on Ser203 by Aurora B during late mitosis has a critical role in regulating cytokinesis. Notably, RASSF1A interacts with Syntaxin16, a member of the t-SNARE family at the midzone and midbody during late mitosis. Aurora B is required for this interaction and for the subsequent recruitment of Syntaxin16 to the midzone and midbody, a prerequisite for the successful completion of cytokinesis. Furthermore, Aurora B depletion results in a failure of Syntaxin16 to properly localize to the midzone and midbody, a mislocalization that was prevented by overexpression of the phosphomimetic RASSF1A (S203D) mutant. Finally, either depletion of Syntaxin]6 or expression of the nonphosphorylatable RASSF1A (S203A) mutant results in cytokinesis defects. Our findings implicate Aurora B-mediated phosphorylation of RASSF1A in the regulation of cytokinesis. [Cancer Res 2009;69(22):8540-4] | - |
dc.description.sponsorship | This work was supported by 21st Century Frontier Functional Human Genome Project and the Korea National Cancer Center Control Program. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | AMER ASSOC CANCER RESEARCH | - |
dc.subject | PROGRESSION | - |
dc.subject | POLES | - |
dc.subject | CELLS | - |
dc.title | Aurora B-Mediated Phosphorylation of RASSF1A Maintains Proper Cytokinesis by Recruiting Syntaxin16 to the Midzone and Midbody | - |
dc.type | Article | - |
dc.identifier.wosid | 000271839200003 | - |
dc.identifier.scopusid | 2-s2.0-72249088778 | - |
dc.type.rims | ART | - |
dc.citation.volume | 69 | - |
dc.citation.issue | 22 | - |
dc.citation.beginningpage | 8540 | - |
dc.citation.endingpage | 8544 | - |
dc.citation.publicationname | CANCER RESEARCH | - |
dc.identifier.doi | 10.1158/0008-5472.CAN-09-1554 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Lim, Dae-Sik | - |
dc.contributor.nonIdAuthor | Song, Min Sup | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | PROGRESSION | - |
dc.subject.keywordPlus | POLES | - |
dc.subject.keywordPlus | CELLS | - |
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