ARF6 and EFA6A regulate the development and maintenance of dendritic spines

The cellular and molecular mechanisms underlying the development and maintenance of dendritic spines are not fully understood. ADP-ribosylation factor 6 ( ARF6) is a small GTPase known to regulate actin remodeling and membrane traffic. Here, we report involvement of ARF6 and exchange factor for ARF6 ( EFA6A) in the regulation of spine development and maintenance. An active form of ARF6 promotes the formation of dendritic spines at the expense of filopodia. EFA6A promotes spine formation in an ARF6 activation-dependent manner. Knockdown of ARF6 and EFA6A by small interfering RNA decreases spine formation. Live imaging indicates that ARF6 knockdown decreases the conversion of filopodia to spines and the stability of early spines. The spine-promoting effect of ARF6 is partially blocked by Rac1. ARF6 and EFA6A protect mature spines from inactivity-induced destabilization. These results suggest that ARF6 and EFA6A may regulate the conversion of filopodia to spines and the stability of both early and mature spines.
Publisher
SOC NEUROSCIENCE
Issue Date
2006-05
Language
ENG
Keywords

HIPPOCAMPAL PYRAMIDAL NEURONS; NUCLEOTIDE-EXCHANGE PROTEIN; ADP-RIBOSYLATION FACTOR-6; MORPHOLOGICAL PLASTICITY; SYNAPTIC PLASTICITY; ACTIN CYTOSKELETON; SMALL GTPASES; MEMBRANE; RHO; RAC

Citation

JOURNAL OF NEUROSCIENCE, v.26, no.18, pp.4811 - 4819

ISSN
0270-6474
DOI
10.1523/JNEUROSCI.4182-05.2006
URI
http://hdl.handle.net/10203/1483
Appears in Collection
BS-Journal Papers(저널논문)
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