Direct recovery of intracellular proteins from Candida utilis using reverse micelles in combination with reducing agent

Abstract

Intracellular proteins were directly extracted from Candida utilis using reverse micelles in combination with a reducing agent. Extraction at pH 10 using 80 mM cetyl trimethyl ammonium bromide (CTAB) and 0.2 % 2-mercaptoethanol was the most favorable condition for cell permeabilization and protein solubilization. Stripping at pH 7 in the presence of 0.5 M KCl resulted in the efficient recovery of solubilized proteins.