DIRECT RECOVERY OF INTRACELLULAR PROTEINS FROM CANDIDA-UTILIS USING REVERSE MICELLES IN COMBINATION WITH A REDUCING AGENT

Intracellular proteins were directly extracted from Candida utilis using reverse micelles in combination with a reducing agent. Extraction at pH 10 using 80 mM cetyl trimethyl ammonium bromide (CTAB) and 0.2 % 2-mercaptoethanol was the most favorable condition for cell permeabilization and protein solubilization. Stripping at pH 7 in the presence of 0.5 M KCl resulted in the efficient recovery of solubilized proteins.
Publisher
CHAPMAN HALL LTD
Issue Date
1993-08
Language
ENG
Keywords

PURIFICATION; EXTRACTION

Citation

BIOTECHNOLOGY TECHNIQUES, v.7, no.8, pp.545 - 550

ISSN
0951-208X
URI
http://hdl.handle.net/10203/13000
Appears in Collection
CBE-Journal Papers(저널논문)
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