Biodegradable polymeric microspheres with "open/closed" pores for sustained release of human growth hormone

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A new approach for attaining Sustained release of protein is introduced, involving a pore-closing process of preformed porous PLGA microspheres. Highly porous biodegradable poly(D,L-lactic-co-glycolic acid) (PLGA) microspheres were fabricated by a single water-in-oil emulsion solvent evaporation technique using Pluronic F127 as an extractable porogen. Recombinant human growth hormone (rhGH) was incorporated into porous microspheres by a simple solution dipping method. For their controlled release, porous microspheres containing hGH were treated with water-miscible solvents in aqueous phase for production of pore-closed microspheres. These microspheres showed Sustained release patterns over an extended period; however, the drug loading efficiency was extremely low. To overcome the drug loading problem, the pore-closing process was performed in an ethanol vapor phase using a fluidized bed reactor. The resultant pore-closed microspheres exhibited high protein loading amount as well as sustained rhGH release profiles. Also, the released rhGH exhibited structural integrity after the treatment. (c) 2006 Elsevier B.V. All rights reserved.
Publisher
ELSEVIER SCIENCE BV
Issue Date
2006-05
Language
English
Article Type
Article
Keywords

POLY(D,L-LACTIC-CO-GLYCOLIC ACID) MICROSPHERES; VITRO PROTEIN RELEASE; FLUIDIZED-BED REACTOR; ORGANIC-SOLVENTS; DRUG-DELIVERY; DEGRADATION; MECHANISM; MICROENCAPSULATION; MICROPARTICLES; FORMULATION

Citation

JOURNAL OF CONTROLLED RELEASE, v.112, no.2, pp.167 - 174

ISSN
0168-3659
DOI
10.1016/j.jconrel.2006.02.004
URI
http://hdl.handle.net/10203/12341
Appears in Collection
NT-Journal Papers(저널논문)BS-Journal Papers(저널논문)
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