Differential induction of autophagy in caspase-3/7 down-regulating and Bcl-2 overexpressing recombinant CHO cells subjected to sodium butyrate treatment

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Previous research showed that co-down-regulation of caspase-3/7 in rCHO cells, unlike Bcl-2 overexpression, did not effectively block apoptotic cell death induced by 3 mM sodium butyrate (NaBu). Here, it is found that the control of autophagy is also related to this different response to NaBu treatment. With NaBu treatment, co-down-regulation of caspase-3/7 enhanced autophagy induction, whereas Bcl-2 overexpression delayed onset of autophagy induction in a Beclin-1 independent manner. The blockage of autophagy showed a detrimental effect on cell viability even in the Bcl-2 overexpressing cells, which suggests the importance of autophagy control for successful anti-cell death engineering of rCHO cells. (C) 2012 Published by Elsevier B.V.
Publisher
ELSEVIER SCIENCE BV
Issue Date
2012-09
Language
English
Article Type
Article
Keywords

HAMSTER OVARY CELLS; APOPTOSIS; DEATH; CULTURE; THROMBOPOIETIN; MITOCHONDRIA; CROSSTALK; PROTEINS; CLEAVAGE; SURVIVAL

Citation

JOURNAL OF BIOTECHNOLOGY, v.161, no.1, pp.34 - 41

ISSN
0168-1656
DOI
10.1016/j.jbiotec.2012.05.011
URI
http://hdl.handle.net/10203/103933
Appears in Collection
BS-Journal Papers(저널논문)
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