Insertion mechanism of cell-penetrating peptides into supported phospholipid membranes revealed by X-ray and neutron reflection

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X-Ray and neutron reflectivity measurements on systems composed of a 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) bilayer and transcription-activating-factor derived peptides (TDPs) have allowed us to determine the mechanism of membrane translocation. By monitoring the structural changes of the bilayers caused by the binding of TDPs while systemically varying temperature and TDP concentration, our results revealed the detailed molecular structures of the stepwise interactions that occurred during the translocation of TDP across the lipid bilayers. While little indication of membrane perturbation was observed at low TDP concentrations, we found that the TDP movement across the membrane induced defect formations in the membrane at higher TDP concentrations.
Publisher
ROYAL SOC CHEMISTRY
Issue Date
2012-08
Language
English
Article Type
Article
Keywords

HUMAN IMMUNODEFICIENCY VIRUS; TAT PEPTIDE; PROTEIN; HIV-1; DELIVERY; DOMAINS; CARGO

Citation

SOFT MATTER, v.8, no.32, pp.8294 - 8297

ISSN
1744-683X
DOI
10.1039/c2sm25913c
URI
http://hdl.handle.net/10203/103649
Appears in Collection
PH-Journal Papers(저널논문)
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