Isolation and characterization of starch-hydrolyzing Pseudoalteromonas sp. A-3 from the coastal sea water of Daecheon, Republic of Korea 대한민국 대천 해안에서 분리한 전분 분해능을 갖는 Pseudoalteromonas sp. A-3 균주의 특징 및 동정

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Strain A-3, an amylase-producing bacteria, was isolated from coastal seawater near Daecheon in the Republic of Korea. It was seen to possess a single polar flagella and grow well, on ASW-YP agar plates, at temperatures of between 20-37°C. However, it grew more slowly at the temperatures of 15°C and 40°C. Similarly, it was observed to grow abundantly, in an Artificial Sea Water-Yeast extract-Peptone (ASW-YP) liquid medium, in a pH range of 6-9, but not grow at pHs of 4-5 and a pH of 10. Strain A-3 was noted as being close to Pseudoalteromonas phenolica O-BC30 , Pseudoalteromonas luteoviolacea NCIMB1893 , Pseudoalteromonas rubra ATCC29570 , and Pseudoalteromonas byunsanensis FR1199 , with 98.30%, 97.86%, 97.78%, and 97.25%) similarities respectively, in its 16S rRNA sequence. A phylogenetic tree revealed that strain A-3 and P. phenolica O-BC30 belong to a clade. However, strain A-3 differed from P. phenolica O-BC30 in relation to a number of physiological characteristics. Strain A-3 exhibited no growth above 5% NaCl concentrations, no utilization of D-glucose, D-mannose, D-maltose, or D-melibose, and no lipase (C-14) activity. All of these properties strongly indicate that strain A-3 is distant from P. phenolica O-BC30 and thus led us to name it Pseudoalteromonas sp. A-3. Pseudoalteromonas sp. A-3 produces α-amylase throughout growth. Maximal amylase activities of 144.48 U/mL and 149.20 U/mL were seen at pH 7.0 and 37°C, respectively. Pseudoalteromonas sp. A-3's high, stable production of α-amylase in addition to its biochemical features, such as alkali-tolerance, suggest that it is a good candidate for industrial applications. © The Korean Society for Microbiology and Biotechnology.
Publisher
Korean Society for Microbiolog and Biotechnology
Issue Date
2011-12
Language
English
Citation

Korean Journal of Microbiology and Biotechnology, v.39, no.4, pp.317 - 323

ISSN
1598-642X
URI
http://hdl.handle.net/10203/103421
Appears in Collection
CBE-Journal Papers(저널논문)
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