Visualization of plasmid delivery to keratinocytes in mouse and human epidermis

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The accessibility of skin makes it an ideal target organ for nucleic acid-based therapeutics; however, effective patient-friendly delivery remains a major obstacle to clinical utility. A variety of limited and inefficient methods of delivering nucleic acids to keratinocytes have been demonstrated; further advances will require well-characterized reagents, rapid noninvasive assays of delivery, and well-developed skin model systems. Using intravital fluorescence and bioluminescence imaging and a standard set of reporter plasmids we demonstrate transfection of cells in mouse and human xenograft skin using intradermal injection and two microneedle array delivery systems. Reporter gene expression could be detected in individual keratinocytes, in real-time, in both mouse skin as well as human skin xenografts. These studies revealed that non-invasive intravital imaging can be used as a guide for developing gene delivery tools, establishing a benchmark for comparative testing of nucleic acid skin delivery technologies.
Publisher
NATURE PUBLISHING GROUP
Issue Date
2011-11
Language
English
Article Type
Article
Keywords

LASER-SCANNING MICROSCOPY; REFLECTANCE CONFOCAL MICROSCOPY; TRANSDERMAL DRUG-DELIVERY; GENE-EXPRESSION; HUMAN SKIN; NAKED DNA; MICRONEEDLES; THERAPY; MELANOMA; CELLS

Citation

SCIENTIFIC REPORTS, v.1

ISSN
2045-2322
DOI
10.1038/srep00158
URI
http://hdl.handle.net/10203/102712
Appears in Collection
CBE-Journal Papers(저널논문)
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