DC Field | Value | Language |
---|---|---|
dc.contributor.author | Nakamura, Kohei | ko |
dc.contributor.author | Tamaki, Hideyuki | ko |
dc.contributor.author | Kang, Myung Suk | ko |
dc.contributor.author | Mochimaru, Hanako | ko |
dc.contributor.author | Lee, Sung Taik | ko |
dc.contributor.author | Nakamura, Kazunori | ko |
dc.contributor.author | Kamagata, Yoichi | ko |
dc.date.accessioned | 2013-03-12T01:28:32Z | - |
dc.date.available | 2013-03-12T01:28:32Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2011-12 | - |
dc.identifier.citation | MICROBES AND ENVIRONMENTS, v.26, no.4, pp.301 - 306 | - |
dc.identifier.issn | 1342-6311 | - |
dc.identifier.uri | http://hdl.handle.net/10203/100962 | - |
dc.description.abstract | We developed a simple, less laborious method to cultivate and isolate obligate anaerobic microorganisms using a six-well plate together with the AnaeroPack System, designated as the six-well plate method. The cultivation efficiency of this method, based on colony-forming units, colony formation time, and colony size, was evaluated with four authentic obligate anaerobes (two methanogenic archaea and two sulfate-reducing bacteria). The method was found to be comparable to or even better than the roll tube method, a technique that is commonly used at present for the cultivation of obligate anaerobes. Further experiments using 21 representative obligate anaerobes demonstrated that all examined anaerobes (11 methanogens, 5 sulfate- or thiosulfate-reducing bacteria, and 5 syntrophs) could form visible colonies on the six-well plate and that these colonies could be successfully subcultured in fresh liquid media. Using this method, an unidentified sulfate-reducing bacterium was successfully isolated from an environmental sample. | - |
dc.language | English | - |
dc.publisher | JAPANESE SOC MICROBIAL ECOLOGY | - |
dc.subject | MICROBIAL ECOLOGY | - |
dc.subject | METHANOSAETA | - |
dc.subject | DIVERSITY | - |
dc.subject | SEDIMENT | - |
dc.subject | BACTERIA | - |
dc.subject | CULTURE | - |
dc.subject | SYSTEMS | - |
dc.subject | STRAIN | - |
dc.subject | FIELD | - |
dc.subject | LAKE | - |
dc.title | A Six-well Plate Method: Less Laborious and Effective Method for Cultivation of Obligate Anaerobic Microorganisms | - |
dc.type | Article | - |
dc.identifier.wosid | 000297556400004 | - |
dc.identifier.scopusid | 2-s2.0-83055161428 | - |
dc.type.rims | ART | - |
dc.citation.volume | 26 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 301 | - |
dc.citation.endingpage | 306 | - |
dc.citation.publicationname | MICROBES AND ENVIRONMENTS | - |
dc.contributor.localauthor | Lee, Sung Taik | - |
dc.contributor.nonIdAuthor | Nakamura, Kohei | - |
dc.contributor.nonIdAuthor | Tamaki, Hideyuki | - |
dc.contributor.nonIdAuthor | Kang, Myung Suk | - |
dc.contributor.nonIdAuthor | Mochimaru, Hanako | - |
dc.contributor.nonIdAuthor | Nakamura, Kazunori | - |
dc.contributor.nonIdAuthor | Kamagata, Yoichi | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | methanogen | - |
dc.subject.keywordAuthor | sulfate-reducing bacterium (SRB) | - |
dc.subject.keywordAuthor | gellan gum | - |
dc.subject.keywordAuthor | uncultured anaerobes | - |
dc.subject.keywordPlus | MICROBIAL ECOLOGY | - |
dc.subject.keywordPlus | METHANOSAETA | - |
dc.subject.keywordPlus | DIVERSITY | - |
dc.subject.keywordPlus | SEDIMENT | - |
dc.subject.keywordPlus | BACTERIA | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | SYSTEMS | - |
dc.subject.keywordPlus | STRAIN | - |
dc.subject.keywordPlus | FIELD | - |
dc.subject.keywordPlus | LAKE | - |
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