A Six-well Plate Method: Less Laborious and Effective Method for Cultivation of Obligate Anaerobic Microorganisms

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We developed a simple, less laborious method to cultivate and isolate obligate anaerobic microorganisms using a six-well plate together with the AnaeroPack System, designated as the six-well plate method. The cultivation efficiency of this method, based on colony-forming units, colony formation time, and colony size, was evaluated with four authentic obligate anaerobes (two methanogenic archaea and two sulfate-reducing bacteria). The method was found to be comparable to or even better than the roll tube method, a technique that is commonly used at present for the cultivation of obligate anaerobes. Further experiments using 21 representative obligate anaerobes demonstrated that all examined anaerobes (11 methanogens, 5 sulfate- or thiosulfate-reducing bacteria, and 5 syntrophs) could form visible colonies on the six-well plate and that these colonies could be successfully subcultured in fresh liquid media. Using this method, an unidentified sulfate-reducing bacterium was successfully isolated from an environmental sample.
Publisher
JAPANESE SOC MICROBIAL ECOLOGY
Issue Date
2011-12
Language
English
Article Type
Article
Keywords

MICROBIAL ECOLOGY; METHANOSAETA; DIVERSITY; SEDIMENT; BACTERIA; CULTURE; SYSTEMS; STRAIN; FIELD; LAKE

Citation

MICROBES AND ENVIRONMENTS, v.26, no.4, pp.301 - 306

ISSN
1342-6311
URI
http://hdl.handle.net/10203/100962
Appears in Collection
BS-Journal Papers(저널논문)
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